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KMID : 0364219900330040476
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Korean Journal of Zoology
1990 Volume.33 No. 4 p.476 ~ p.492
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Studies on the Properties of lntracelluar Arsenic Binding Substances in the Rat Liver
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Choi Im-Soon
Boo Moon-Jong
Kim Chung-Hyon
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Abstract
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Sodium arsenite (As) aqueous solution containing 4 ppm wss given to male rats for 15 days as drinking water. Electrophoretic pattern of liver cytosol from As-fed rats appeared to be significantly different from that of the control rats. Although the normal protein content of the cytosol fraction of As-fed rat liver was decreased, 8 stress proteins were increased. In liver cytosol fractions of As-treated rat, one kind of arsenic-binding substance (AsBS) was observed. Molecular weight of AsBS was identified to be 500 D and composition of amino acid was glycine, glutamic acid and cysteine. Glutathione (GSH) appeared to bind to arsenic and GSH-As complex showed the same mobility as AsBS on gel filtration chromatography. GSH conjugated As prevented As from inhibiting respiration, conformational change and swelling-contration of mitochondria. According to the above results. it is concluded that in vfuo treated arsenic stimulated synthesis of stress protein, and arsenic-binding substance might be glutathione and have a protective role against arsenic toxicity.
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KEYWORD
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Arsenic, Stress protein, Arsenic-binding substance
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